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The column was eluted isocratically at a flow rate of 450 μL/min for 1 minute at 80% mobile-phase A (10 mM NH 4 Ac/methanol/acetic acid 95:5:0.1, by volume), followed by a linear gradient to 80% mobile-phase B (methanol/acetic acid

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To date, the most studied biomedical applications for these complexes include antibacterial, anticancer, and antifungal activities, although anti-arthritic, anti-inflammatory, anti-osteoporotic, antioxidant, anti-rheumatic, and antiviral properties have also been investigated